Kane out of World Cup warm-up against Japan with injury
The captain, who only joined the squad at the weekend after being rested for the first half of the international camp, has enjoyed a spectacular season for Bayern Munich, scoring 48 goals in 40 matches.
"Harry Kane is rested tonight as a precaution having picked up a minor issue in training, but remains with the squad receiving further assessment," the Football Association said in a statement on X.
England boss Thomas Tuchel, speaking ahead of kick-off at Wembley, said the injury came "from basically out of nothing".